Comparison between batch and fed-batch production of rhamnolipid by Pseudomonas aeruginosa

This paper presents a comparison between batch and three different sets of fed batch fermentations forrhamnolipid production by Pseudomonas aeruginosa. The batch run was performed with 500 ml of culturemedium having the initial glycerol and sodium nitrate concentrations of 30 and 8.3 g/l, respectively. For a fedbatch run with nitrogen source in feed, 250 ml of the nitrogen excluded culture medium was in the bioreactorinitially, and 250 ml culture medium containing 16.6 g/l sodium nitrate was fed to the bioreactor continuously.A similar procedure was repeated for fed batch runs with carbon, and phosphorus source in feed. Statisticalanalysis showed that fed batch runs were better than batch in term of rhamnolipid production, and amongthe fed batch runs the maximum amount of rhamnolipid. (4.12 g Rhamnose Equivalent/l) was for the fedbatch run with the carbon source in feed.